Chromaffin Cells: Methods and Protocols

Part I Culturing and morphology

1. Rat pheochromocytoma PC12 cells in culture

Chin-Tien Wang

2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation

Alejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo

3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells

Keita Harada, Hidetada Matsuoka, Masumi Inoue

4. Transmission electron microscopy: a method for studying the adrenochromaffin cells

Anna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia

5. Immunogold for protein location in chromaffin cells

Rafael Luján, Rocio Alfaro-Ruiz and Carolina Aguado

6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy

Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu

Part II Stimulus-secretion coupling

7. Online detection of catecholamine release from the perfused rat adrenal gland

Ricardo Borges, Ana de la Iglesia and Antonio G. García

8. On-line recording of perifused chromaffin cells

Ricardo De Pascual and Luis Gandía

9. Recording of chromaffin cell electrical activity in situ in acute adrenal slices

Nathali Guerineau

10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats

Tzitzitlini Alejandre-García, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Millán-Aldaco and Arturo Hernández-Cruz.

11. Measurements of calcium in chromaffin cell organelles using targeted aequorins.

Jaime Santo-Domingo, Pilar Álvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier Álvarez

12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements

Muhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg

13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell

Elizabeth P. Seward and Robert C. Wykes

14. Quantal release analysis of electrochemically active molecules using single-cell amperometry

Natalia Domínguez, Pablo Montenegro and J. David Machado

15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs

Giulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli

16. Vesicle collision protocols for the study of quantum size and exocytotic fraction released

Soodabeh Majdi, Alex S. Lima and Andrew Ewing

17. Patch Amperometry and Intracellular Patch Electrochemistry

Eugene V. Mosharov and Manfred Lindau

18. Artificial cells for dissecting exocytosis

Yuanmo Wang and Ann-Sofie Cans

Part III Chromaffin granules and membrane traffic

19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells

Arlek Gonzalez-Jamett, María Constanza Maldifassi, Ana María Cárdenas

20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells

José Villanueva, Yolanda Gimenez-Molina and Luis M. Gutiérrez

21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imagi