Cartilage Tissue Engineering

1. Cartilage Tissue Engineering: An Introduction

Martin J. Stoddart, Elena Della Bella, and Angela R. Armiento

2. Chondrocyte Isolation and Expansion

Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, and Karina Wright

3. Isolation of Chondrons from Hyaline Cartilage

Jasmijn V. Korpershoek, Margot Rikkers, and Lucienne A. Vonk

4. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation

Ilyas M. Khan, Joshua McKenna, and Yadan Zhang

5. Physioxic Culture of Chondrogenic Cells

Girish Pattappa, Brandon D. Markway, Denitsa Docheva, and Brian Johnstone

6. Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Angela R. Armiento, Yann D. Ladner, Elena Della Bella, and Martin J. Stoddart

7. Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation

Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, and Wiltrud Richter

8. Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells

Amanda R. Dicks, Nancy Steward, Farshid Guilak, and Chia-Lung Wu

9. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans

Yann D. Ladner, Mauro Alini, and Angela R. Armiento

10. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy

Alexander Otahal, Andrea De Luna, Ali Mobasheri, and Stefan Nehrer

11. Metabolomic Profiling to Understand Chondrocyte Metabolism

Priyanka P. Brahmachary, Hope D. Welhaven, and Ronald K. June

12. Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes

Huan Meng, Clare L. Thompson, Clarissa Coveney, Angus K. Wann, and Martin M. Knight

13. Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads

Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, and Angela R. Armiento

14. Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis

Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, and Gabriela G. Loots

15. Modulation of MicroRNA Expression during In Vitro Chondrogenesis

Austin Bell-Hensley, Hongjun Zheng, and Audrey McAlinden

16. Purification and Isolation of Proteins from Hyaline Cartilage

Makenna J.