Plant Cell Division: Methods and Protocols

1. Methods to Visualize the Actin Cytoskeleton during Plant Cell Division

Marie-Cécile Caillaud

Part I: Mitosis and Cell Cycle Progression

2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, and Inna Lermontova

3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips

Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, and William F. Thompson

4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures

Kateřina Bisová

5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission

Veronika Kselíková, Vilém Zachleder, and Kateřina Bisová

Part II: Manipulation of Cell Division

6. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy

Lukas Hoermayer, Jiří Friml, and Matous Glanc

7. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells

Tamara Jimenez-Gόngora, Huang Tan, and Rosa Lozano-Durán

Part III: Quantification of Cell Division Patterning

8. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf

Jingjing Fang and Xueyong Li

9. Studying Cell Division Plane Positioning in Early-Stage Embryos

Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia

10. Means to Quantify Vascular Cell File Numbers in Different Tissues

Helena E. Arents, Gugan Eswaran, Matous Glanc, Ari Pekka Mähönen, and Bert De Rybel

11. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis

Roya Campos and Jaimie M. Van Norman

12. Quantification of Cell Division Angles in the Arabidopsis Root

Katia Belcram, David Legland, and Martine Pastuglia

Part IV: Imaging and Quantifying Plant Cytokinesis

13. A Quantitative Method for Evaluating Phragmoplast Morphology

Takema Sasaki and Yoshihisa Oda

14. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis

Alexandra Deli, Leda-Eleni Tympa, and Panagiotis N. Moschou

15. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells

Keisho Maeda and Takumi Higaki

16. Ultrastructural Observation of Cyt